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M9630542.TXT
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1996-02-27
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Document 0542
DOCN M9630542
TI Detection of Toxoplasma gondii by PCR and tissue culture in
cerebrospinal fluid and blood of human immunodeficiency
virus-seropositive patients.
DT 9603
AU Dupon M; Cazenave J; Pellegrin JL; Ragnaud JM; Cheyrou A; Fischer I;
Leng B; Lacut JY; Service de Medecine Interne, Hopital Pellegrin,
Bordeaux,; France.
SO J Clin Microbiol. 1995 Sep;33(9):2421-6. Unique Identifier : AIDSLINE
MED/96025163
AB To investigate whether both tissue culture and PCR on a sequence from
the repetitive rDNA could contribute to the diagnosis of toxoplasmosis,
blood samples and, if they were available, cerebrospinal fluid (CSF) and
aqueous humor samples from 72 human immunodeficiency virus-seropositive
patients with suspected toxoplasmosis were prospectively tested. For 10
patients with fever of unknown origin but without confirmed
toxoplasmosis, no Toxoplasma gondii was detected. For two patients with
confirmed toxoplasmic uveitis, only PCR of aqueous humor samples was
positive. Of 60 patients (48 with CSF samples) with neurological signs,
25 (from 13 of whom CSF samples were available) had confirmed cerebral
toxoplasmosis and 10 had a positive PCR of CSF and/or blood samples,
while for 1 patient culture of the CSF sample was also positive. Unlike
tissue culture, PCR of rDNA is of value for the detection of cerebral
toxoplasmosis in human immunodeficiency virus-seropositive patients,
provided that both CSF and blood samples are available (sensitivity,
76.9%; specificity, 100%).
DE Adolescence Adult Aged Animal Base Sequence Female Human HIV
Seropositivity/COMPLICATIONS/*PARASITOLOGY Male Middle Age Molecular
Sequence Data Polymerase Chain Reaction Predictive Value of Tests
Toxoplasma/*ISOLATION & PURIF
Toxoplasmosis/COMPLICATIONS/DIAGNOSIS/*PARASITOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).